Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 log unit reduction of viable cells when administered at 2.5 μM. The effectiveness of GD11 against P. aeruginosa biofilms grown in flow-cells and microtiter trays was also demonstrated. Confocal laser scanning microscopy of flow-cell-grown biofilms suggests that the treatment has a biocidal effect against bacterial biofilm cells.

Antimicrobial and anti-biofilm effect of a novel BODIPY photosensitizer against Pseudomonas aeruginosa PAO1

ORLANDI, VIVIANA TERESA;CARUSO, ENRICO;BANFI, STEFANO;BARBIERI, PAOLA
2014-01-01

Abstract

Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 log unit reduction of viable cells when administered at 2.5 μM. The effectiveness of GD11 against P. aeruginosa biofilms grown in flow-cells and microtiter trays was also demonstrated. Confocal laser scanning microscopy of flow-cell-grown biofilms suggests that the treatment has a biocidal effect against bacterial biofilm cells.
2014
http://www.tandfonline.com/doi/full/10.1080/08927014.2014.940921
BODIPY; photodynamic treatment; Pseudomonas aeruginosa
Orlandi, VIVIANA TERESA; Rybtke, M.; Caruso, Enrico; Banfi, Stefano; Tolker Nielsen, T.; Barbieri, Paola
File in questo prodotto:
File Dimensione Formato  
2014_Antimicrobial and anti-biofilm effect of a novel BODIPY.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: DRM non definito
Dimensione 346.55 kB
Formato Adobe PDF
346.55 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/1966320
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 38
  • ???jsp.display-item.citation.isi??? 35
social impact