Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1receptor in tissue slices from rodent brain.

Assay of GTPγS binding in autoradiography

GABAGLIO, MARINA;PRINI, PAMELA;ZAMBERLETTI, ERICA;RUBINO, TIZIANA;PAROLARO, DANIELA
2016-01-01

Abstract

Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1receptor in tissue slices from rodent brain.
2016
Humana Press Inc.
978-1-4939-3537-6
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2042156
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