Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1receptor in tissue slices from rodent brain.
Assay of GTPγS binding in autoradiography
GABAGLIO, MARINA;PRINI, PAMELA;ZAMBERLETTI, ERICA;RUBINO, TIZIANA;PAROLARO, DANIELA
2016-01-01
Abstract
Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1receptor in tissue slices from rodent brain.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
