The mature egg of fishes is surrounded by an acellular coat conventionally called the chorion. In Carassius auratus three main layers can be recognized at the electron microscopic level, and we have termed them ch1, ch2, and ch3 starting from the outer side to the inner side close to the egg plasma membrane. Isolated chorion from mature ovarian and ovulated eggs can be obtained by homogenization and further purification techniques. We analyzed these preparations on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and at least 20 polypeptide chains were reproducibly observed. The solubilization conditions were tested by means of various agents under reducing and nonreducing conditions. The present report focuses on the temporal and spatial pathway of chorion formation during oocyte growth, and we report the solubilization and partial characterization of protein and glycoprotein components of purified chorions. © 1988.

STRUCTURE AND COMPOSITION OF THE FISH EGG CHORION (CARASSIUS-AURATUS)

BRIVIO, MAURIZIO FRANCESCO
1988

Abstract

The mature egg of fishes is surrounded by an acellular coat conventionally called the chorion. In Carassius auratus three main layers can be recognized at the electron microscopic level, and we have termed them ch1, ch2, and ch3 starting from the outer side to the inner side close to the egg plasma membrane. Isolated chorion from mature ovarian and ovulated eggs can be obtained by homogenization and further purification techniques. We analyzed these preparations on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and at least 20 polypeptide chains were reproducibly observed. The solubilization conditions were tested by means of various agents under reducing and nonreducing conditions. The present report focuses on the temporal and spatial pathway of chorion formation during oocyte growth, and we report the solubilization and partial characterization of protein and glycoprotein components of purified chorions. © 1988.
Cotelli, ; F., Andronico; Brivio, MAURIZIO FRANCESCO
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/13264
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