Actin was identified in boar and mole spermatozoa by utilizing indirect immunofiuorescence, immunoelectron microscopy, and SDS-PAGE, followed by blot and screening with an anti-actin monoclonal antibody. Actin was detected in two places in the sperm head: the equatorial segment of the acrosome and the postacrosomal region. The protein was present in a nonfilamentous form and was localized under the plasma membrane. A small amount of actin was also detected in the sperm tail. The function of actin in the sperm head is discussed. © 1986.
Autori: | |
Data di pubblicazione: | 1986 |
Titolo: | LOCALIZATION AND DISTRIBUTION OF ACTIN IN MAMMALIAN SPERM HEADS |
Rivista: | JOURNAL OF ULTRASTRUCTURE AND MOLECULAR STRUCTURE RESEARCH |
Digital Object Identifier (DOI): | 10.1016/0889-1605(86)90003-0 |
Codice identificativo ISI: | WOS:A1986K177200002 |
Codice identificativo Scopus: | 2-s2.0-0022741066 |
Codice identificativo Pubmed: | 3316422 |
Appare nelle tipologie: | Articolo su Rivista |
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