Actin was identified in boar and mole spermatozoa by utilizing indirect immunofiuorescence, immunoelectron microscopy, and SDS-PAGE, followed by blot and screening with an anti-actin monoclonal antibody. Actin was detected in two places in the sperm head: the equatorial segment of the acrosome and the postacrosomal region. The protein was present in a nonfilamentous form and was localized under the plasma membrane. A small amount of actin was also detected in the sperm tail. The function of actin in the sperm head is discussed. © 1986.
LOCALIZATION AND DISTRIBUTION OF ACTIN IN MAMMALIAN SPERM HEADS
BRIVIO, MAURIZIO FRANCESCO
1986-01-01
Abstract
Actin was identified in boar and mole spermatozoa by utilizing indirect immunofiuorescence, immunoelectron microscopy, and SDS-PAGE, followed by blot and screening with an anti-actin monoclonal antibody. Actin was detected in two places in the sperm head: the equatorial segment of the acrosome and the postacrosomal region. The protein was present in a nonfilamentous form and was localized under the plasma membrane. A small amount of actin was also detected in the sperm tail. The function of actin in the sperm head is discussed. © 1986.
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