The renin-angiotensin system plays a key role in the regulation of cardiovascular functions and in particular angiotensin II type I receptor (AT(1)R)-operated pathways are involved in the modulation of inflammation in the vascular wall. In the present study we assessed the pattern of expression of AT(1)Rs on different human circulating leukocyte subsets. Venous blood was obtained from healthy male subjects. Leukocyte subsets were purified by immunomagnetic cell sorting or identified in whole blood using multiparametric cytometric analysis. RT-PCR analysis showed that AT(1)R mRNA was expressed in polymorphonuclear leukocytes (PMNs), monocytes, B-lymphocytes, and, to a lesser extent, T-lymphocytes. Flow cytometric analysis revealed that the frequency of expression of AT(1)Rs was: PN/FNs > monocytes >= B-lymphocytes >> T-lymphocytes, while receptor density per positive cells was: PMNs >= B-lymphocytes > T-lymphocytes >= monocytes. AT(1)Rs are expressed on PMNs, monocytes, T- and B-lymphocytes, however the expression pattern is peculiar to each subset, possibly suggesting distinct roles in the various cell types. Investigating the expression and the functional role of AT(1)Rs on circulating leukocyte subsets, as well as their possible modifications in disease conditions before and after pharmacological treatments, is likely to provide novel clues to the comprehension of the mechanisms involved in the therapeutic efficacy of currently available agents.

Angiotensin II type 1 receptor expression on human leukocyte subsets: a flow cytometric and RT-PCR study

RASINI, EMANUELA;COSENTINO, MARCO;MARINO, FRANCA;LEGNARO, MASSIMILIANO;FERRARI, MARCO;GUASTI, LUIGINA;VENCO, ACHILLE;LECCHINI, SERGIO
2006-01-01

Abstract

The renin-angiotensin system plays a key role in the regulation of cardiovascular functions and in particular angiotensin II type I receptor (AT(1)R)-operated pathways are involved in the modulation of inflammation in the vascular wall. In the present study we assessed the pattern of expression of AT(1)Rs on different human circulating leukocyte subsets. Venous blood was obtained from healthy male subjects. Leukocyte subsets were purified by immunomagnetic cell sorting or identified in whole blood using multiparametric cytometric analysis. RT-PCR analysis showed that AT(1)R mRNA was expressed in polymorphonuclear leukocytes (PMNs), monocytes, B-lymphocytes, and, to a lesser extent, T-lymphocytes. Flow cytometric analysis revealed that the frequency of expression of AT(1)Rs was: PN/FNs > monocytes >= B-lymphocytes >> T-lymphocytes, while receptor density per positive cells was: PMNs >= B-lymphocytes > T-lymphocytes >= monocytes. AT(1)Rs are expressed on PMNs, monocytes, T- and B-lymphocytes, however the expression pattern is peculiar to each subset, possibly suggesting distinct roles in the various cell types. Investigating the expression and the functional role of AT(1)Rs on circulating leukocyte subsets, as well as their possible modifications in disease conditions before and after pharmacological treatments, is likely to provide novel clues to the comprehension of the mechanisms involved in the therapeutic efficacy of currently available agents.
AT(1)Rs, cardiovascular disease, immune cells, inflammation
Rasini, Emanuela; Cosentino, Marco; Marino, Franca; Legnaro, Massimiliano; Ferrari, Marco; Guasti, Luigina; Venco, Achille; Lecchini, Sergio
File in questo prodotto:
File Dimensione Formato  
Regulatory_Peptides_2006.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: DRM non definito
Dimensione 437.61 kB
Formato Adobe PDF
437.61 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/1496766
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 16
  • Scopus 43
  • ???jsp.display-item.citation.isi??? 41
social impact