Full-grown frog ovarian oocytes (Rana esculenta), were voltage clamped with a conventional two-microelectrode system. Depolarizations from a holding potential of--60 mV produced slowly developing outward currents. Two-step clamp experiments showed that, in Ringer's solution, this current has a reversal potential at about--84 mV. Substitution of either sodium or chloride with impermeant ions in the external solution did not alter significantly the activation of the current nor its reversal potential. Increasing the potassium ions concentration caused a shift on the reversal potential in the positive direction with a slope of about 48 mV per decade. The presence of TEA ions (50 mM) in the external solution partially reduced the current. It is concluded that the membrane of full-grown frog ovarian oocytes possesses voltage-dependent ionic channels permeated mainly by potassium. They appear to play an important role in the control of membrane potential.
|Data di pubblicazione:||1985|
|Titolo:||A voltage-dependent K+ channel controlling the membrane potential in frog oocytes.|
|Digital Object Identifier (DOI):||10.1007/BF00583279|
|Codice identificativo ISI:||WOS:A1985AHJ0700007|
|Codice identificativo Scopus:||2-s2.0-0021914609|
|Codice identificativo Pubmed:||2580268|
|Appare nelle tipologie:||Articolo su Rivista|