The interaction between different HOradical dot radical scavengers in a three-component antioxidant system has been investigated by means of 19F-NMR spectroscopy. This system is composed of bovine serum albumin (BSA), trolox, and N-(4-hydroxyphenyl)-trifluoroacetamide (CF3PAF). The antioxidant capacity of BSA and trolox has been assessed by measuring the amount of trifluoroacetamide (TFAM) arising from the radical mediated decomposition of CF3PAF. When assayed separately, both trolox and BSA behaved as antioxidants, as they were effective to protect CF3PAF from HOradical dot radical-mediated decomposition. By contrast, trolox enhanced the production of TFAM in the presence of BSA, thus behaving as a pro-oxidant. Urate, carnosine, glucose, and propylgallate showed antioxidant properties both with or without BSA. CF3PAF and trolox were found to bind to BSA with association constants in the order of 5 × 103 M−1 and to compete for the same binding sites. These results have been discussed in terms of BSA-catalysed cross-reactions between trolox-derived secondary radicals and CF3PAF.
The interaction between different HO. radical scavengers in a three-component antioxidant system has been investigated by means of 19F-NMR spectroscopy. This system is composed of bovine serum albumin (BSA), trolox, and N-(4-hydroxyphenyl)-trifluoroacetamide (CF3PAF). The antioxidant capacity of BSA and trolox has been assessed by measuring the amount of trifluoroacetamide (TFAM) arising from the radical mediated decomposition of CF3PAF. When assayed separately, both trolox and BSA behaved as antioxidants, as they were effective to protect CF3PAF from HO. radical-mediated decomposition. By contrast, trolox enhanced the production of TFAM in the presence of BSA, thus behaving as a pro-oxidant. Urate, carnosine, glucose, and propylgallate showed antioxidant properties both with or without BSA. CF3PAF and trolox were found to bind to BSA with association constants in the order of 5×103M-1 and to compete for the same binding sites. These results have been discussed in terms of BSA-catalysed cross-reactions between trolox-derived secondary radicals and CF3PAF. © 2003 Elsevier Inc. All rights reserved.
Modulation of the antioxidant activity of HO scavengers by albumin binding: a 19F-NMR study
FASANO, MAURO
2003-01-01
Abstract
The interaction between different HO. radical scavengers in a three-component antioxidant system has been investigated by means of 19F-NMR spectroscopy. This system is composed of bovine serum albumin (BSA), trolox, and N-(4-hydroxyphenyl)-trifluoroacetamide (CF3PAF). The antioxidant capacity of BSA and trolox has been assessed by measuring the amount of trifluoroacetamide (TFAM) arising from the radical mediated decomposition of CF3PAF. When assayed separately, both trolox and BSA behaved as antioxidants, as they were effective to protect CF3PAF from HO. radical-mediated decomposition. By contrast, trolox enhanced the production of TFAM in the presence of BSA, thus behaving as a pro-oxidant. Urate, carnosine, glucose, and propylgallate showed antioxidant properties both with or without BSA. CF3PAF and trolox were found to bind to BSA with association constants in the order of 5×103M-1 and to compete for the same binding sites. These results have been discussed in terms of BSA-catalysed cross-reactions between trolox-derived secondary radicals and CF3PAF. © 2003 Elsevier Inc. All rights reserved.
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