Dopaminergic neurons are particularly exposed to oxidative stress because dopamine metabolism gives rise to endogenous toxins. Here, two different models for dopamine toxicity have been investigated using proteomics: stable α-synuclein-expressing neuroblastoma (SH-SY5Y) cells to understand how α-synuclein modulates dopamine toxicity at the central level, and cultured T-cell leukaemia (Jurkat) cells challenged with dopamine or l-dopa to evaluate protein changes on the surface of peripheral cells. α-synuclein-expressing cells were more resistant compared with wild-type cells, showing upregulation of antioxidant proteins including the Parkinson's disease-related DJ-1 protein. In addition, treatment of prototypical T-cells with l-dopa induced significant changes in proteins on the cell surface, indicating that peripheral blood lymphocytes may be good indicators of dopamine toxicity. © 2008 Elsevier Ltd. All rights reserved.

Proteomics as a tool to investigate cell models for dopamine toxicity

FASANO, MAURO;ALBERIO, TIZIANA;COLAPINTO, MONICA;MILA, SILVIA;
2008

Abstract

Dopaminergic neurons are particularly exposed to oxidative stress because dopamine metabolism gives rise to endogenous toxins. Here, two different models for dopamine toxicity have been investigated using proteomics: stable α-synuclein-expressing neuroblastoma (SH-SY5Y) cells to understand how α-synuclein modulates dopamine toxicity at the central level, and cultured T-cell leukaemia (Jurkat) cells challenged with dopamine or l-dopa to evaluate protein changes on the surface of peripheral cells. α-synuclein-expressing cells were more resistant compared with wild-type cells, showing upregulation of antioxidant proteins including the Parkinson's disease-related DJ-1 protein. In addition, treatment of prototypical T-cells with l-dopa induced significant changes in proteins on the cell surface, indicating that peripheral blood lymphocytes may be good indicators of dopamine toxicity. © 2008 Elsevier Ltd. All rights reserved.
http://dx.doi.org/10.1016/j.parkreldis.2008.04.016
α-Synuclein; Dopamine; Human neuroblastoma; Human T-cell leukaemia; Parkinson's disease; Proteomics;
Fasano, Mauro; Alberio, Tiziana; Colapinto, Monica; Mila, Silvia; L., Lopiano
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/1714961
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 12
  • ???jsp.display-item.citation.isi??? ND
social impact