Human serum heme-albumin (HSA-heme) displays globin-like properties. Here, the allosteric inhibition of ferric heme [heme-Fe(III)] binding to human serum albumin (HSA) and of ferric HSA-heme [HSA-heme-Fe(III)]-mediated peroxynitrite isomerization by isoniazid and rifampicin is reported. Moreover, the allosteric inhibition of isoniazid and rifampicin binding to HSA by heme-Fe(III) has been investigated. Data were obtained at pH 7.2 and 20.0 °C. The affinity of isoniazid and rifampicin for HSA [K (0) = (3.9 ± 0.4) × 10(-4) and (1.3 ± 0.1) × 10(-5) M, respectively] decreases by about 1 order of magnitude upon heme-Fe(III) binding to HSA [K (h) = (4.3 ± 0.4) × 10(-3) and (1.2 ± 0.1) × 10(-4) M, respectively]. As expected, the heme-Fe(III) affinity for HSA [H (0) = (1.9 ± 0.2) × 10(-8) M] decreases by about 1 order of magnitude in the presence of saturating amounts of isoniazid and rifampicin [H (d) = (2.1 ± 0.2) × 10(-7) M]. In the absence and presence of CO(2), the values of the second-order rate constant (l (on)) for peroxynitrite isomerization by HSA-heme-Fe(III) are 4.1 × 10(5) and 4.3 × 10(5) M(-1) s(-1), respectively. Moreover, isoniazid and rifampicin inhibit dose-dependently peroxynitrite isomerization by HSA-heme-Fe(III) in the absence and presence of CO(2). Accordingly, isoniazid and rifampicin impair in a dose-dependent fashion the HSA-heme-Fe(III)-based protection of free L: -tyrosine against peroxynitrite-mediated nitration. This behavior has been ascribed to the pivotal role of Tyr150, a residue that either provides a polar environment in Sudlow's site I (i.e., the binding pocket of isoniazid and rifampicin) or protrudes into the heme-Fe(III) cleft, depending on ligand binding to Sudlow's site I or to the FA1 pocket, respectively. These results highlight the role of drugs in modulating heme-Fe(III) binding to HSA and HSA-heme-Fe(III) reactivity.

Isoniazid and rifampicin inhibit allosterically heme binding to albumin and peroxynitrite isomerization by heme-albumin.

FANALI, GABRIELLA;FASANO, MAURO
2011-01-01

Abstract

Human serum heme-albumin (HSA-heme) displays globin-like properties. Here, the allosteric inhibition of ferric heme [heme-Fe(III)] binding to human serum albumin (HSA) and of ferric HSA-heme [HSA-heme-Fe(III)]-mediated peroxynitrite isomerization by isoniazid and rifampicin is reported. Moreover, the allosteric inhibition of isoniazid and rifampicin binding to HSA by heme-Fe(III) has been investigated. Data were obtained at pH 7.2 and 20.0 °C. The affinity of isoniazid and rifampicin for HSA [K (0) = (3.9 ± 0.4) × 10(-4) and (1.3 ± 0.1) × 10(-5) M, respectively] decreases by about 1 order of magnitude upon heme-Fe(III) binding to HSA [K (h) = (4.3 ± 0.4) × 10(-3) and (1.2 ± 0.1) × 10(-4) M, respectively]. As expected, the heme-Fe(III) affinity for HSA [H (0) = (1.9 ± 0.2) × 10(-8) M] decreases by about 1 order of magnitude in the presence of saturating amounts of isoniazid and rifampicin [H (d) = (2.1 ± 0.2) × 10(-7) M]. In the absence and presence of CO(2), the values of the second-order rate constant (l (on)) for peroxynitrite isomerization by HSA-heme-Fe(III) are 4.1 × 10(5) and 4.3 × 10(5) M(-1) s(-1), respectively. Moreover, isoniazid and rifampicin inhibit dose-dependently peroxynitrite isomerization by HSA-heme-Fe(III) in the absence and presence of CO(2). Accordingly, isoniazid and rifampicin impair in a dose-dependent fashion the HSA-heme-Fe(III)-based protection of free L: -tyrosine against peroxynitrite-mediated nitration. This behavior has been ascribed to the pivotal role of Tyr150, a residue that either provides a polar environment in Sudlow's site I (i.e., the binding pocket of isoniazid and rifampicin) or protrudes into the heme-Fe(III) cleft, depending on ligand binding to Sudlow's site I or to the FA1 pocket, respectively. These results highlight the role of drugs in modulating heme-Fe(III) binding to HSA and HSA-heme-Fe(III) reactivity.
2011
http://dx.doi.org/10.1007/s00775-010-0706-2
Binding Sites; drug effects, Heme; antagonists /&/ inhibitors/chemistry, Humans, Isoniazid; chemistry/pharmacology, Models; Molecular, Peroxynitrous Acid; chemistry, Rifampin; chemistry/pharmacology, Serum Albumin; antagonists /&/ inhibitors/chemistry, Structure-Activity Relationship
P., Ascenzi; A., Bolli; A. d., Masi; G. R., Tundo; Fanali, Gabriella; M., Coletta; Fasano, Mauro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/1722184
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