One of the deleterious effect of oxidative stress found in sea bass larvae is the appearance of dystrophic lesions in the epaxial musculature due to the ingestion of high DHA diets, especially when vitamin E levels are low (Betancor et al., 2011; Betancor et al., submitted). Diverse antioxidant enzymes (AOE) prevent the cascade of oxidant reactions, intercepting and inactivating with the reactive intermediates, closing the lipid-peroxidation cycle. This defence system includes enzymes such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) (Winston Halliwell and Gutteridge, 1996). Together with these enzymes dietary micronutrients such as vitamin E and C, carotenoids or selenium play important roles in antioxidant defences in fish. Adequate supplementation of marine fish larvae with antioxidants is therefore an important issue in diet formulation in order to avoid in vivo lipid peroxidation. The purpose of the present study was to investigate if diets supplemented with Se (5mg kg-1 DW) and vitamin E protects sea bass larvae muscle from oxidative stress when high levels of DHA are included in diets. To reach this objective, growth, survival, TBARS, fatty acid profile, α-tocopherol and selenium contents and mRNA expression of CAT, SOD, GPX, IGF-I and II and myosin heavy chain (MyHC) were determined.
Dietary selenium inclusion decreases oxidative stress indicators and muscle injuries in sea bass larvae fed high DHA microdiets.
TEROVA, GENCIANA;
2011-01-01
Abstract
One of the deleterious effect of oxidative stress found in sea bass larvae is the appearance of dystrophic lesions in the epaxial musculature due to the ingestion of high DHA diets, especially when vitamin E levels are low (Betancor et al., 2011; Betancor et al., submitted). Diverse antioxidant enzymes (AOE) prevent the cascade of oxidant reactions, intercepting and inactivating with the reactive intermediates, closing the lipid-peroxidation cycle. This defence system includes enzymes such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) (Winston Halliwell and Gutteridge, 1996). Together with these enzymes dietary micronutrients such as vitamin E and C, carotenoids or selenium play important roles in antioxidant defences in fish. Adequate supplementation of marine fish larvae with antioxidants is therefore an important issue in diet formulation in order to avoid in vivo lipid peroxidation. The purpose of the present study was to investigate if diets supplemented with Se (5mg kg-1 DW) and vitamin E protects sea bass larvae muscle from oxidative stress when high levels of DHA are included in diets. To reach this objective, growth, survival, TBARS, fatty acid profile, α-tocopherol and selenium contents and mRNA expression of CAT, SOD, GPX, IGF-I and II and myosin heavy chain (MyHC) were determined.
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