The aim of our present research is to produce mutant forms of d-amino acid oxidase from Rhodotorula gracilis in order to determine d-amino acid content in different biological samples. During the past few years, our group has produced yeast d-amino acid oxidase variants with altered substrate specificity (e.g., active on acidic, or hydrophobic, or on all d-amino acids) both by rational design and directed evolution methods. Now, the kinetic constants for a number of amino acids (even for unnatural ones) of the most relevant d-amino acid oxidase variants have been investigated. This information constitutes the basis for considering potential analytical applications in this important field.

Analyzing the d-amino acid content in biological samples by engineered enzymes

FRATTINI, LUCA FRANCO;ROSINI, ELENA;POLLEGIONI, LOREDANO;PILONE, MIRELLA
2011

Abstract

The aim of our present research is to produce mutant forms of d-amino acid oxidase from Rhodotorula gracilis in order to determine d-amino acid content in different biological samples. During the past few years, our group has produced yeast d-amino acid oxidase variants with altered substrate specificity (e.g., active on acidic, or hydrophobic, or on all d-amino acids) both by rational design and directed evolution methods. Now, the kinetic constants for a number of amino acids (even for unnatural ones) of the most relevant d-amino acid oxidase variants have been investigated. This information constitutes the basis for considering potential analytical applications in this important field.
http://dx.doi.org/10.1016/j.jchromb.2011.02.036
D-Amino acids; Detection; Flavoproteins; Mutagenesis; Oxidases; Substrate specificity
Frattini, LUCA FRANCO; Rosini, Elena; Pollegioni, Loredano; Pilone, Mirella
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/1730197
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