The substrate preferences of three acylases two wild-type enzymes and an evolved variant obtained by directed evolution - which are prototypical enzymes for glutaryl-7-ACA acylase and cephalosporin C acylase subfamilies, have been investigated. A preliminary screening of enzymes' performances on a large set of substrates has been carried out by a colorimetric assay performed in 96-well plates and by a pH-Star monitoring the hydrolytic activities. Subsequently, kinetic data for selected substrates have been determined, thus elucidating the substrate preference of members of glutaryl-7-ACA acylase vs. cephalosporin C acylase subfamilies. These achievements pave the way to the ability of choosing the best enzyme for the hydrolysis of different compounds of industrial importance.
|Data di pubblicazione:||2012|
|Titolo:||On the substrate preference of glutaryl acylases|
|Rivista:||JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC|
|Digital Object Identifier (DOI):||10.1016/j.molcatb.2011.12.001|
|Codice identificativo ISI:||WOS:000301033300008|
|Codice identificativo Scopus:||2-s2.0-84855733503|
|Appare nelle tipologie:||Articolo su Rivista|