Zona-free mouse eggs are amenable to patch recording as well as to whole-cell recording (Bland et al., 1984; Peres, 1986a). Although many patches are silent, others show spontaneous channel opening; the single channel current is 1.5 pA and inwardly directed at resting potential (RP). Current amplitudes distribute normally as a single population at RP, however at RP-50 mV the amplitude histogram indicates two channel populations. Open time distribution is exponential with a mean open time between 4 and 7 msec at RP. In three cases out of thirty-eight, in which depolarizations were given from a holding potential of RP-50 mV, microscopic currents very similar in kinetics and voltage-dependence to the whole-cell Ca2+ current were observed. No single-channel currents could be resolved in these traces. The results reported here indicate that the voltage-dependent Ca2+ current of the mouse egg goes through low-conductance channels located in high density spots on the egg surface.
|Data di pubblicazione:||1987|
|Titolo:||Single- and multi-channel currents recorded with patch electrodes in mouse eggs.|
|Rivista:||CELL BIOLOGY INTERNATIONAL REPORTS|
|Digital Object Identifier (DOI):||10.1016/0309-1651(87)90082-8|
|Codice identificativo ISI:||A1987H971800007|
|Codice identificativo Pubmed:||2441882|
|Appare nelle tipologie:||Articolo su Rivista|