Using the whole-cell technique, we have measured recurring hyperpolarizations induced by fetal calf serum and bradykinin in human fibroblasts. By coupling fura-2 microfluorimetry to electrophysiology, we have also measured directly cytosolic Ca2+ and found that Ca2+ oscillations occur in synchrony with membrane currents. Mitogen stimulation of cells in which intracellular K+ had been replaced with Cs+ resulted in the abolishment of the outward current. We conclude then that the mitogen-induced recurring hyperpolarizations in human fibroblasts are due to the opening of Ca2(+)-activated K+ channels.

Mitogen-induced oscillations of membrane potential and Ca2+ in human fibroblasts.

GIOVANNARDI, STEFANO
1990-01-01

Abstract

Using the whole-cell technique, we have measured recurring hyperpolarizations induced by fetal calf serum and bradykinin in human fibroblasts. By coupling fura-2 microfluorimetry to electrophysiology, we have also measured directly cytosolic Ca2+ and found that Ca2+ oscillations occur in synchrony with membrane currents. Mitogen stimulation of cells in which intracellular K+ had been replaced with Cs+ resulted in the abolishment of the outward current. We conclude then that the mitogen-induced recurring hyperpolarizations in human fibroblasts are due to the opening of Ca2(+)-activated K+ channels.
1990
2001
(Fibroblast); Cytoplasmic Ca2+; Hyperpolarization; K+ channel; Mitogen; Oscillation
Peres, Antonio; Giovannardi, Stefano
File in questo prodotto:
File Dimensione Formato  
FEBS Letters - February 12 1990 - Peres.pdf

non disponibili

Tipologia: Versione Editoriale (PDF)
Licenza: Copyright dell'editore
Dimensione 383.01 kB
Formato Adobe PDF
383.01 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/1754288
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 15
social impact