Proteins which, on the basis of their solubility in 0.35% NaCl-2% TCA and of their electrophoretic mobility, correspond to animal HMG 1/2 family were isolated from nuclei of ungerminated pea embryos. These proteins ound with a high degree of specificity to synthetic cruciform DNA produced by annealing chemically synthesized oligonucleotides. Hence, specific binding to four-way junction DNA, previously reported for animal HMG 1 and 2 proteins proved also to be a property of plant HMG 1/2 family, in spite of their low homology to the animal ones.

Specific interaction of plant HMG-like proteins with cruciform DNA

BRACALE, MARCELLA;
1994-01-01

Abstract

Proteins which, on the basis of their solubility in 0.35% NaCl-2% TCA and of their electrophoretic mobility, correspond to animal HMG 1/2 family were isolated from nuclei of ungerminated pea embryos. These proteins ound with a high degree of specificity to synthetic cruciform DNA produced by annealing chemically synthesized oligonucleotides. Hence, specific binding to four-way junction DNA, previously reported for animal HMG 1 and 2 proteins proved also to be a property of plant HMG 1/2 family, in spite of their low homology to the animal ones.
1994
Pisum sativum; chromosomal proteins; cruciform DNA; high mobility group proteins
Bracale, Marcella; Galli, M. G.; Savini, C.; Bianchi, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/1770523
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