The rapid development of nanotechnology is causing an ubiquitously presence of nanoparticles (NPs) in the environment. Cobalt, iron and nickel zerovalent NPs are the most promising materials for nanotechnological applications due to their application in fields such as electronics, communication and security systems, food industry and cosmetics, environmental remediation, water purification, tools for diagnosis and treatment of diseases and even in army. It is evident that a multidisciplinary study of the possible toxicity of the above mentioned NPs is fundamental. For this reason we have performed in vitro experiments on SKOV-3 and U87 cell lines to evaluate NP cytotoxicity, uptake and their influence on gene expression. The results have shown a higher cytotoxicity of nickel and cobalt compare to iron. All NP are readily internalized into cells within 30 min exposure; this observation is in agreement with the cytotoxicity, that seems to be dose but not time dependent. Furthermore, results of Real-Time PCR suggest that Fe-NPs and Co-NPs, at the tested concentration, have moderate effects on the expression, while Ni-NPs seems to up-regulate the investigate genes.
Nanoparticle toxicity
PEDRETTI, ELISA;ROSSI, FEDERICA;GORNATI, ROSALBA;BERNARDINI, GIOVANNI BATTISTA
2012-01-01
Abstract
The rapid development of nanotechnology is causing an ubiquitously presence of nanoparticles (NPs) in the environment. Cobalt, iron and nickel zerovalent NPs are the most promising materials for nanotechnological applications due to their application in fields such as electronics, communication and security systems, food industry and cosmetics, environmental remediation, water purification, tools for diagnosis and treatment of diseases and even in army. It is evident that a multidisciplinary study of the possible toxicity of the above mentioned NPs is fundamental. For this reason we have performed in vitro experiments on SKOV-3 and U87 cell lines to evaluate NP cytotoxicity, uptake and their influence on gene expression. The results have shown a higher cytotoxicity of nickel and cobalt compare to iron. All NP are readily internalized into cells within 30 min exposure; this observation is in agreement with the cytotoxicity, that seems to be dose but not time dependent. Furthermore, results of Real-Time PCR suggest that Fe-NPs and Co-NPs, at the tested concentration, have moderate effects on the expression, while Ni-NPs seems to up-regulate the investigate genes.
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