Electrophoretic patterns of isozymes, allowing the direct study of gene products, represent a valid tool in detecting genetic and epigenetic variations both involved in determining somaclonal variation. In the present work, three isozyme systems, glutamate-oxalacetate transaminase (GOT, EC 2.6.1.1), alcohol dehydrogenase (ADH, EC 1.1.1.1), and esterase (EST, EC 3.1.1.1), were analyzed in different organs of callus-regenerated plants of potato and on the tubers of the first vegetative reproduction. As concerns ADH and GOT, the electrophoretic patterns showed the disappearance of one band in may regenerated plants in respect to the parental cultivar. This loss of allele expression can be determined by a stable (ADH) or by an unstable change (GOT) as suggested by the permanence or not of the variation in the tubers. The electrofocusing of esterase revealed the presence of additional bands in respect of parental cultivar. Taking into account that somaclonal variation might arise as the response of the genome to a shock caused by tissue culture, it is suggested that sequence rearrangements represent likely mechanisms respectively involved in the loss or in the appearance of gene expression.

ISOZYME VARIATION IN LEAF-CALLUS REGENERATED PLANTS OF SOLANUM-TUBEROSUM

VANNINI, CANDIDA
1987-01-01

Abstract

Electrophoretic patterns of isozymes, allowing the direct study of gene products, represent a valid tool in detecting genetic and epigenetic variations both involved in determining somaclonal variation. In the present work, three isozyme systems, glutamate-oxalacetate transaminase (GOT, EC 2.6.1.1), alcohol dehydrogenase (ADH, EC 1.1.1.1), and esterase (EST, EC 3.1.1.1), were analyzed in different organs of callus-regenerated plants of potato and on the tubers of the first vegetative reproduction. As concerns ADH and GOT, the electrophoretic patterns showed the disappearance of one band in may regenerated plants in respect to the parental cultivar. This loss of allele expression can be determined by a stable (ADH) or by an unstable change (GOT) as suggested by the permanence or not of the variation in the tubers. The electrofocusing of esterase revealed the presence of additional bands in respect of parental cultivar. Taking into account that somaclonal variation might arise as the response of the genome to a shock caused by tissue culture, it is suggested that sequence rearrangements represent likely mechanisms respectively involved in the loss or in the appearance of gene expression.
1987
somaclonal variation, isozymes, potato
Allicchio, R.; Antonioli, C.; Graziani, L.; Roncarati, R.; Vannini, Candida
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/1790969
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