European sea bass (Dicentrarchus labrax) is the second most cultured fish species in the Mediterranean area. It is therefore important to establish appropriate feeding management regimes and to gain better knowledge on nutritional requirements for this aquacultured species, exploring not only new feed ingredients and feeding strategies, but also, understanding in depth the molecular mechanisms regulating feed digestion, utilization and metabolism of nutrients. Transcriptomic analysis has become a useful nutrigenomic discovery tool for identifying the molecular basis of biological responses to nutrition and novel nutritional biomarkers in fish. The aim of the present study was to evaluate how the transcriptional activity of genes controlling lipid metabolism in sea bass are modulated in a tissue-specific manner in response to a fasting period, followed by refeeding. The study was focused on liver, white skeletal muscle, brain and adipose tissue and included a panel of 30 genes in which desaturases, elongases, triacylglycerol lipases, fatty acid binding proteins, β-oxidation and oxidative phosphorylation enzymes, phospholipid-related enzymes and lipid transcription factors were represented.
The changing gene expression profile induced by fasting and refeeding in European sea bass Dicentrarchus labrax.
RIMOLDI, SIMONA;TEROVA, GENCIANA;
2014-01-01
Abstract
European sea bass (Dicentrarchus labrax) is the second most cultured fish species in the Mediterranean area. It is therefore important to establish appropriate feeding management regimes and to gain better knowledge on nutritional requirements for this aquacultured species, exploring not only new feed ingredients and feeding strategies, but also, understanding in depth the molecular mechanisms regulating feed digestion, utilization and metabolism of nutrients. Transcriptomic analysis has become a useful nutrigenomic discovery tool for identifying the molecular basis of biological responses to nutrition and novel nutritional biomarkers in fish. The aim of the present study was to evaluate how the transcriptional activity of genes controlling lipid metabolism in sea bass are modulated in a tissue-specific manner in response to a fasting period, followed by refeeding. The study was focused on liver, white skeletal muscle, brain and adipose tissue and included a panel of 30 genes in which desaturases, elongases, triacylglycerol lipases, fatty acid binding proteins, β-oxidation and oxidative phosphorylation enzymes, phospholipid-related enzymes and lipid transcription factors were represented.
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