Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.

Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics

BONDI, HEATHER;MONTI, CHIARA;ALBERIO, TIZIANA;FASANO, MAURO;
2016

Abstract

Mitochondria possess a proteolytic system that contributes to the regulation of mitochondrial dynamics, mitochondrial biogenesis and mitophagy. We aimed at the identification by bottom-up proteomics of altered protein processing due to the activation of mitochondrial proteases in a cellular model of impaired dopamine homeostasis. Moreover, we optimized the conditions for top-down proteomics to identify the cleavage site sequences.
http://www.journals.elsevier.com/eupa-open-proteomics/
Electroelution; Mitochondria; Protease; Shotgun; Top-down; Biochemistry
Di Pierro, Alice; Bondi, Heather; Monti, Chiara; Pieroni, Luisa; Cilio, Enrico; Urbani, Andrea; Alberio, Tiziana; Fasano, Mauro; Ronci, Maurizio
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/2049714
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