Bioengineered organs raised in vitro are candidate substitutes for natural organs in biological, pharmacological and clinical applications. We have studied cell kinetics in a human skin equivalent (HSE) using a combined immunohistochemical and flow cytometric approach. Morphological analysis has shown that, relative to unstimulated natural skin, cell proliferation mainly occurs in the basal layer of the epidermal equivalent. Immunohistochemical and flow cytometric measurements of the growth fraction suggested a cell turnover comparable to that of natural skin. Immunohistochemical labelling indices matched well with flow cytometric data. These observations are consistent with morphological and histochemical data demonstrating normal cell differentiation and tissue architecture in HSE and suggest that such HSE may be a usefull substitute for human skin.

Cell kinetics in a model of artificial skin. An immunohistochemical and flow cytometric analysis

CASASCO, MARCO;ZERBINATI, NICOLA;CALLIGARO, ALBERTO
2001

Abstract

Bioengineered organs raised in vitro are candidate substitutes for natural organs in biological, pharmacological and clinical applications. We have studied cell kinetics in a human skin equivalent (HSE) using a combined immunohistochemical and flow cytometric approach. Morphological analysis has shown that, relative to unstimulated natural skin, cell proliferation mainly occurs in the basal layer of the epidermal equivalent. Immunohistochemical and flow cytometric measurements of the growth fraction suggested a cell turnover comparable to that of natural skin. Immunohistochemical labelling indices matched well with flow cytometric data. These observations are consistent with morphological and histochemical data demonstrating normal cell differentiation and tissue architecture in HSE and suggest that such HSE may be a usefull substitute for human skin.
Cell Culture Techniques; Cell Death; Cells, Cultured; Dermis; Epidermis; Fibroblasts; Flow Cytometry; Humans; Immunoenzyme Techniques; Keratinocytes; Ki-67 Antigen; Biocompatible Materials; Models, Biological
Casasco, A; Casasco, Marco; Cornaglia, A. I; Zerbinati, Nicola; Mazzini, G; Calligaro, Alberto
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/2063981
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