Adolescence represents a vulnerable period for the psychiatric consequences of delta9-tetrahydrocannabinol (Delta(9)-THC) exposure, however, the molecular underpinnings of this vulnerability remain to be established. Histone modifications are emerging as important epigenetic mechanisms involved in the etiopathogenesis of psychiatric diseases, thus, we investigated the impact of chronic Delta(9)-THC exposure on histone modifications in different brain areas of female rats. We checked histone modifications associated to both transcriptional repression (H3K9 di- and tri-methylation, H3K27 tri-methylation) and activation (H3K9 and H3K14 acetylation) after adolescent and adult chronic Delta(9)-THC exposure in the hippocampus, nucleus accumbens, and amygdala. Chronic exposure to increasing doses of Delta(9)-THC for 11 days affected histone modifications in a region- and age-specific manner. The primary effect in the adolescent brain was represented by changes leading to transcriptional repression, whereas the one observed after adult treatment led to transcriptional activation. Moreover, only in the adolescent brain, the primary effect was followed by a homeostatic response to counterbalance the Delta(9)-THC-induced repressive effect, except in the amygdala. The presence of a more complex response in the adolescent brain may be part of the mechanisms that make the adolescent brain vulnerable to Delta(9)-THC adverse effects.

Chronic Delta(9)-THC Exposure Differently Affects Histone Modifications in the Adolescent and Adult Rat Brain

PRINI, PAMELA;PENNA, FEDERICA;ALBERIO, TIZIANA;RUBINO, TIZIANA
2017

Abstract

Adolescence represents a vulnerable period for the psychiatric consequences of delta9-tetrahydrocannabinol (Delta(9)-THC) exposure, however, the molecular underpinnings of this vulnerability remain to be established. Histone modifications are emerging as important epigenetic mechanisms involved in the etiopathogenesis of psychiatric diseases, thus, we investigated the impact of chronic Delta(9)-THC exposure on histone modifications in different brain areas of female rats. We checked histone modifications associated to both transcriptional repression (H3K9 di- and tri-methylation, H3K27 tri-methylation) and activation (H3K9 and H3K14 acetylation) after adolescent and adult chronic Delta(9)-THC exposure in the hippocampus, nucleus accumbens, and amygdala. Chronic exposure to increasing doses of Delta(9)-THC for 11 days affected histone modifications in a region- and age-specific manner. The primary effect in the adolescent brain was represented by changes leading to transcriptional repression, whereas the one observed after adult treatment led to transcriptional activation. Moreover, only in the adolescent brain, the primary effect was followed by a homeostatic response to counterbalance the Delta(9)-THC-induced repressive effect, except in the amygdala. The presence of a more complex response in the adolescent brain may be part of the mechanisms that make the adolescent brain vulnerable to Delta(9)-THC adverse effects.
http://www.mdpi.com/1422-0067/18/10/2094/pdf
Adolescents; Adults; Amygdala; Hippocampus; Histone modifications; Nucleus accumbens; Δ; 9; -THC;
Prini, Pamela; Penna, Federica; Sciuccati, E.; Alberio, Tiziana; Rubino, Tiziana
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/2066388
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