In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely untapped by using traditional isolation and cultivation methods. Metagenomics, which is culture independent, represents a powerful tool for discovering novel enzymes from unculturable microorganisms. Herein, we summarize the variety of approaches adopted for mining environmental DNA and, based on a systematic literature review, we provide a comprehensive list of 332 industrially relevant enzymes discovered from metagenomes within the last three years.

Metagenomics: novel enzymes from non-culturable microbes

Berini, Francesca
;
Casciello, Carmine;Marcone, Giorgia Letizia;Marinelli, Flavia
2017-01-01

Abstract

In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely untapped by using traditional isolation and cultivation methods. Metagenomics, which is culture independent, represents a powerful tool for discovering novel enzymes from unculturable microorganisms. Herein, we summarize the variety of approaches adopted for mining environmental DNA and, based on a systematic literature review, we provide a comprehensive list of 332 industrially relevant enzymes discovered from metagenomes within the last three years.
2017
environmental libraries; heterologous expression; industrial enzymes; metagenomics; screening
Berini, Francesca; Casciello, Carmine; Marcone, Giorgia Letizia; Marinelli, Flavia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2066516
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