Erythropoietin Increases GABA A Currents in Human Cortex from TLE Patients

Erythropoietin (EPO)is a hematopoietic growth factor that has an important role in the erythropoiesis. EPO and its receptor (EPO-R)are expressed all over in the mammalian brain. Furthermore, it has been reported that EPO may exert neuroprotective effect in animal models of brain disorders as ischemia and epilepsy. Here, we investigate whether EPO could modulate the GABA-evoked currents (I GABA )in both human epileptic and non-epileptic control brain tissues. Therefore, we transplanted in Xenopus oocytes cell membranes obtained from autoptic and surgical brain tissues (cortex)of seven temporal lope epilepsy (TLE)patients and of five control patients. Two microelectrodes voltage-clamp technique has been used to record I GABA . Moreover, qRT-PCR assay was performed in the same human tissues to quantify the relative gene expression levels of EPO/EPO-R. To further confirm experiments in oocytes, we performed additional experiments using patch-clamp recording in slices obtained from rat cerebellum. We show that exposure to EPO significantly increased the amplitude of the I GABA in all the patients analyzed. No differences in the expression of EPO and EPO-R in both TLE and control patients have been found. Notably, the increase of I GABA has been recorded also in rat cerebellar slices. Our findings show a new modulatory action of EPO on GABA A receptors (GABA A -Rs). This effect could be relevant to balance the GABAergic dysfunction in human TLE.