Aim: To demonstrate that cobalt nanoparticles doses are safe for use in humans and to understand the consequences of the particulate effects, which may persist inside the cells. Materials & methods: Human adipose stem cells were used. We evaluated cell recovery by viability test, morphology and ultrastructure using electronic and optical microscopy, while gene expression was assessed utilizing real-time PCR. Results: After exposure, most stem cells recovered their normal function. Co3O4-nanoparticles remained inside the cell for the entirety of the considered time. A slight modification of gene expression was observed in the exposed cells. Conclusion: After exposure to 100 M cobalt nanoparticles, most cells returned to normal function. Nanoparticle toxicity was due to ions released by dissolution as well as from the nanoparticles themselves.

Recovery ability of human adipose stem cells exposed to cobalt nanoparticles: outcome of dissolution

Marina Borgese;Federica Rossi;Luigi Valdatta;Giovanni Bernardini;Rosalba Gornati
2020

Abstract

Aim: To demonstrate that cobalt nanoparticles doses are safe for use in humans and to understand the consequences of the particulate effects, which may persist inside the cells. Materials & methods: Human adipose stem cells were used. We evaluated cell recovery by viability test, morphology and ultrastructure using electronic and optical microscopy, while gene expression was assessed utilizing real-time PCR. Results: After exposure, most stem cells recovered their normal function. Co3O4-nanoparticles remained inside the cell for the entirety of the considered time. A slight modification of gene expression was observed in the exposed cells. Conclusion: After exposure to 100 M cobalt nanoparticles, most cells returned to normal function. Nanoparticle toxicity was due to ions released by dissolution as well as from the nanoparticles themselves.
cell morphology; inductively coupled plasma mass spectrometry; molecular biomarkers; nanotoxicology; TEM;
Borgese, Marina; Rossi, Federica; Bonfanti, Patrizia; Colombo, Anita; Mantecca, Paride; Valdatta, Luigi; Bernardini, GIOVANNI BATTISTA; Gornati, Rosalba
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/2094015
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