Objectives: This study analyzed salivary samples of COVID-19 patients and compared the results with their clinical and laboratory data. Methods: Salivary samples of 25 COVID-19 patients were analyzed by rRT-PCR. The following data were collected: age, sex, comorbidities, drugs. Lactate dehydrogenase (LDH) and ultrasensitive reactive C protein (usRCP) values were registered on the same day when a salivary swab was collected. Prevalence of positivity in saliva and association between clinical data and the cycle threshold as a semiquantitative indicator of viral load were considered. Results: Twenty-five subjects were recruited into this study, 17 males and 8 females. The mean age was 61.5 +/− 11.2 years. Cardiovascular and/or dysmetabolic disorders were observed in 65.22% of cases. All the samples tested positive for the presence of SARS-CoV-2, while there was an inverse association between LDH and Ct values. Two patients showed positive salivary results on the same days when their pharyngeal or respiratory swabs showed conversion. Conclusions: Saliva is a reliable tool to detect SARS-CoV-2. The role of saliva in COVID-19 diagnosis could not be limited to a qualitative detection of the virus, but it may also provide information about the clinical evolution of the disease.

Saliva is a reliable tool to detect SARS-CoV-2

Lorenzo Azzi
Primo
;
Giulio Carcano
Secondo
;
Francesco Gianfagna;Paolo Grossi;Daniela Dalla Gasperina;Angelo Genoni;Mauro Fasano;Fausto Sessa;Lucia Tettamanti;Vittorio Maurino;Angelo Tagliabue
Penultimo
;
Andreina Baj
Ultimo
2020-01-01

Abstract

Objectives: This study analyzed salivary samples of COVID-19 patients and compared the results with their clinical and laboratory data. Methods: Salivary samples of 25 COVID-19 patients were analyzed by rRT-PCR. The following data were collected: age, sex, comorbidities, drugs. Lactate dehydrogenase (LDH) and ultrasensitive reactive C protein (usRCP) values were registered on the same day when a salivary swab was collected. Prevalence of positivity in saliva and association between clinical data and the cycle threshold as a semiquantitative indicator of viral load were considered. Results: Twenty-five subjects were recruited into this study, 17 males and 8 females. The mean age was 61.5 +/− 11.2 years. Cardiovascular and/or dysmetabolic disorders were observed in 65.22% of cases. All the samples tested positive for the presence of SARS-CoV-2, while there was an inverse association between LDH and Ct values. Two patients showed positive salivary results on the same days when their pharyngeal or respiratory swabs showed conversion. Conclusions: Saliva is a reliable tool to detect SARS-CoV-2. The role of saliva in COVID-19 diagnosis could not be limited to a qualitative detection of the virus, but it may also provide information about the clinical evolution of the disease.
2020
Coronavirus; COVID-19; nCoV-2019; Saliva; SARS-CoV-2
Azzi, Lorenzo; Carcano, Giulio; Gianfagna, Francesco; Grossi, PAOLO ANTONIO; DALLA GASPERINA, Daniela; Genoni, ANGELO PAOLO; Fasano, Mauro; Sessa, Fau...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2095224
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