Hydrotalcites-like compounds (HTlc) also known as anionic clays or layered double hydroxides, represent a class of lamellar solids with net positive charge balanced by exchangeable interlayer anions1. HTlc are excellent materials for designing drug delivery systems due to their biocompatibility, ability to intercalate different anionic species, pH-dependent stability and low toxicity2. The present study focuses on the preparation of HTlc intercalating separately two different molecules: the anticancer drug norcantharidin (NCTD), which is known for inducing cell cycle arrest at G2/M phase and the tetra-sulfonated aluminum phtalocyanine (AlPcS4), a photosensitizer used in photodynamic therapy3. The obtained hybrid ZnAl HTlc were characterized in terms of X-Ray powder diffraction pattern, thermogravimetric analysis, SEM microscopy, drug release profile, in vitro cytotoxicity and ability to produce ROS and 1O2 upon light irradiation. Our results clearly indicate that the two selected compounds are efficiently intercalated within HTlc layers. Moreover, in vitro preliminary studies on a panel of cancer cells lines indicated a greater cytotoxicity of the two drugs once loaded on HTlc either when administrated singularly or in combination. In addition, the analysis of the synergistic effect of the two formulations was evaluated by determining their combination index, which showed a greater cytotoxicity when using as a 1:2 ratio of AlPcS4@HTlc and NCTD@HTlc, respectively. 1. V. Ambrogi, L. Perioli, M. Ricci, L. Pulcini, M. Nocchetti, S. Giovagnoli, C. Rossi, Microporous Mesoporous Mater. 2008, 115, 405. 2. T. Posati, F. Bellezza, L. Tarpani, S. Perni, L. Latterini, V. Marsili, A. Cipiciani, Appl. Clay Sci. 2012, 55, 62. 3. C. Martini, C. Ferroni, M. B. Gariboldi, A. Donnadio, A. Aluigi, G. Sotgiu, F. Liscio, P. Dambruoso, M. L. Navacchia, T. Posati, G. Varchi, ACS Appl. Nano Mater. 2018, 1, 11, 6387-6397.
Intercalation of Bioactive Molecules into Nanosized ZnAl Hydrotalcites for Combined Chemo and Photo Cancer Treatment
Marzia Bruna Gariboldi;
2019-01-01
Abstract
Hydrotalcites-like compounds (HTlc) also known as anionic clays or layered double hydroxides, represent a class of lamellar solids with net positive charge balanced by exchangeable interlayer anions1. HTlc are excellent materials for designing drug delivery systems due to their biocompatibility, ability to intercalate different anionic species, pH-dependent stability and low toxicity2. The present study focuses on the preparation of HTlc intercalating separately two different molecules: the anticancer drug norcantharidin (NCTD), which is known for inducing cell cycle arrest at G2/M phase and the tetra-sulfonated aluminum phtalocyanine (AlPcS4), a photosensitizer used in photodynamic therapy3. The obtained hybrid ZnAl HTlc were characterized in terms of X-Ray powder diffraction pattern, thermogravimetric analysis, SEM microscopy, drug release profile, in vitro cytotoxicity and ability to produce ROS and 1O2 upon light irradiation. Our results clearly indicate that the two selected compounds are efficiently intercalated within HTlc layers. Moreover, in vitro preliminary studies on a panel of cancer cells lines indicated a greater cytotoxicity of the two drugs once loaded on HTlc either when administrated singularly or in combination. In addition, the analysis of the synergistic effect of the two formulations was evaluated by determining their combination index, which showed a greater cytotoxicity when using as a 1:2 ratio of AlPcS4@HTlc and NCTD@HTlc, respectively. 1. V. Ambrogi, L. Perioli, M. Ricci, L. Pulcini, M. Nocchetti, S. Giovagnoli, C. Rossi, Microporous Mesoporous Mater. 2008, 115, 405. 2. T. Posati, F. Bellezza, L. Tarpani, S. Perni, L. Latterini, V. Marsili, A. Cipiciani, Appl. Clay Sci. 2012, 55, 62. 3. C. Martini, C. Ferroni, M. B. Gariboldi, A. Donnadio, A. Aluigi, G. Sotgiu, F. Liscio, P. Dambruoso, M. L. Navacchia, T. Posati, G. Varchi, ACS Appl. Nano Mater. 2018, 1, 11, 6387-6397.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
