Background: The introduction of nucleic acid amplification technology (NAT) for screening pooled or individual donations remarkably improved the safety of blood products. The size of mini-pooled NAT is considered critical for identification of HIV-1 infected donors during preseroconversion phase of infection. We describe a case of HIV-1 infection in a serologically negative repeat blood donor identified by 16 minipool (MP) NAT. Materials and Methods: The donation was tested by Roche Cobas AmpliScreen HIV-1 Test with manual extraction (MultiPrep Specimen Processing Procedure). The sensitivity of different MP sizes was observed. Serial samples of infected donor were examined with different third and fourth generation HIV-1 serological assays. Results: In the index donation viral load was 515 copies/ml corresponding to about 50 IU when diluted in 16 MP. Abbott third and fourth generation EIA tests detected the seroconversion four days later the index donation. Conclusion: The report emphasizes the relevance of a very small size of MP to really reduce the window serologic phase of current EIA test by HIV-1 NAT test. © 2005 Blackwell Publishing.

HIV-1 NAT minipool during the pre-seroconversion window period: Detection of a repeat blood donor

Maggi, F.;
2006-01-01

Abstract

Background: The introduction of nucleic acid amplification technology (NAT) for screening pooled or individual donations remarkably improved the safety of blood products. The size of mini-pooled NAT is considered critical for identification of HIV-1 infected donors during preseroconversion phase of infection. We describe a case of HIV-1 infection in a serologically negative repeat blood donor identified by 16 minipool (MP) NAT. Materials and Methods: The donation was tested by Roche Cobas AmpliScreen HIV-1 Test with manual extraction (MultiPrep Specimen Processing Procedure). The sensitivity of different MP sizes was observed. Serial samples of infected donor were examined with different third and fourth generation HIV-1 serological assays. Results: In the index donation viral load was 515 copies/ml corresponding to about 50 IU when diluted in 16 MP. Abbott third and fourth generation EIA tests detected the seroconversion four days later the index donation. Conclusion: The report emphasizes the relevance of a very small size of MP to really reduce the window serologic phase of current EIA test by HIV-1 NAT test. © 2005 Blackwell Publishing.
2006
HIV EIA sensitivity; HIV-1 NAT; Individual NAT; Minipool NAT; Preseroconversion period; Adult; HIV Seropositivity; Humans; Male; RNA; Viral; Blood Donors; HIV-1; Reverse Transcriptase Polymerase Chain Reaction; Viral Load; Hematology
Palla, P.; Vatteroni, M. L.; Vacri, L.; Maggi, F.; Baicchi, U.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2102360
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