Objectives: To detect possible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA contamination of inanimate surfaces in areas at high risk of aerosol formation by patients with coronavirus disease 2019 (COVID-19). Methods: Sampling was performed in the emergency unit and the sub-intensive care ward. SARS-CoV-2 RNA was extracted from swabbed surfaces and objects and subjected to real-time RT-PCR targeting RNA-dependent RNA polymerase and E genes. Virus isolation from positive samples was attempted in vitro on Vero E6 cells. Results: Twenty-six samples were collected and only two were positive for low-level SARS-CoV-2 RNA, both collected on the external surface of continuous positive airway pressure helmets. All transport media were inoculated onto susceptible cells, but none induced a cytopathic effect on day 7 of culture. Conclusions: Even though daily contact with inanimate surfaces and patient fomites in contaminated areas may be a medium of infection, our data obtained in real-life conditions suggest that it might be less extensive than hitherto recognized.

Severe acute respiratory syndrome coronavirus 2 RNA contamination of inanimate surfaces and virus viability in a health care emergency unit

Corbella M.;Paolucci S.;Novazzi F.;
2020-01-01

Abstract

Objectives: To detect possible severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA contamination of inanimate surfaces in areas at high risk of aerosol formation by patients with coronavirus disease 2019 (COVID-19). Methods: Sampling was performed in the emergency unit and the sub-intensive care ward. SARS-CoV-2 RNA was extracted from swabbed surfaces and objects and subjected to real-time RT-PCR targeting RNA-dependent RNA polymerase and E genes. Virus isolation from positive samples was attempted in vitro on Vero E6 cells. Results: Twenty-six samples were collected and only two were positive for low-level SARS-CoV-2 RNA, both collected on the external surface of continuous positive airway pressure helmets. All transport media were inoculated onto susceptible cells, but none induced a cytopathic effect on day 7 of culture. Conclusions: Even though daily contact with inanimate surfaces and patient fomites in contaminated areas may be a medium of infection, our data obtained in real-life conditions suggest that it might be less extensive than hitherto recognized.
2020
Coronavirus disease 2019; Environmental contamination; Severe acute respiratory syndrome coronavirus-2; Surfaces; Vero E6 cells; Animals; Betacoronavirus; Chlorocebus aethiops; Coronavirus Envelope Proteins; Equipment Contamination; Fomites; Humans; Intensive Care Units; Microbial Viability; RNA-Dependent RNA Polymerase; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2; Vero Cells; Viral Envelope Proteins; Viral Proteins
Colaneri, M.; Seminari, E.; Novati, S.; Asperges, E.; Biscarini, S.; Piralla, A.; Percivalle, E.; Cassaniti, I.; Baldanti, F.; Bruno, R.; Mondelli, M. U.; Brunetti, E.; Di Matteo, A.; Maiocchi, L.; Zuccaro, V.; Pagnucco, L.; Ludovisi, S.; Lissandrin, R.; Parisi, A.; Sacchi, P.; Patruno, S. F. A.; Michelone, G.; Gulminetti, R.; Zanaboni, D.; Maserati, R.; Orsolini, P.; Vecchia, M.; Di Filippo, A.; Sambo, M.; Lupi, M.; Roda, S.; Pieri, T. C.; Gallazzi, I.; Sachs, M.; Valsecchi, P.; Perlini, S.; Alfano, C.; Bonzano, M.; Briganti, F.; Crescenzi, G.; Falchi, A. G.; Guarnone, R.; Guglielmana, B.; Maggi, E.; Martino, I.; Pettenazza, P.; di Marco, S. P.; Quaglia, F.; Sabena, A.; Salinaro, F.; Speciale, F.; Zunino, I.; de Lorenzo, M.; Secco, G.; Dimitry, L.; Cappa, G.; Maisak, I.; Chiodi, B.; Sciarrini, M.; Barcella, B.; Resta, F.; Moroni, L.; Vezzoni, G.; Scattaglia, L.; Boscolo, E.; Zattera, C.; Fidel, T. M.; Vincenzo, C.; Vignaroli, D.; Bazzini, M.; Iotti, G.; Mojoli, F.; Belliato, M.; Perotti, L.; Mongodi, S.; Tavazzi, G.; Marseglia, G.; Licari, A.; Brambilla, I.; Barbarini, D.; Bruno, A.; Cambieri, P.; Campanini, G.; Cavanna, C.; Comolli, G.; Corbella, M.; Daturi, R.; Furione, M.; Mariani, B.; Marone, P.; Monzillo, V.; Paolucci, S.; Parea, M.; Rovida, F.; Sarasini, A.; Zavattoni, M.; Adzasehoun, G.; Ardizzone, M.; Bellotti, L.; Brunco, V.; Cabano, E.; Casali, G.; Capella, L.; Devitis, D.; Dossena, L.; Frisco, G.; Garbagnoli, G.; Gardellini, F.; Girello, A.; Guerrizio, A.; Landini, V.; Lucchelli, C.; Maliardi, V.; Piemontese, P.; Pezzaia, S.; Premoli, M.; Rebuffa, C.; Bagnarino, J.; Bergami, F.; Bonetti, A.; Caneva, G.; Corcione, A.; Di Martino, R.; Di Napoli, A.; Ferrari, A.; Ferrari, G.; Fiorina, L.; Gallone, A.; Giardina, F.; Girardi, A.; Mercato, A.; Novazzi, F.; Ratano, G.; Rossi, B.; Saverimpilla, G.; Sciabica, I. M.; Tallarita, M.; Nepita, E. V.; Vitali, J.; Cerino, A.; Varchetta, S.; Oliviero, B.; Mantovani, S.; Mele, D.; Calvi, M.; Tizzoni, M.; Nicola, C.; Triarico, A.; Petronella, V.; Marena, C.; Muzzi, A.; Lago, P.; Comandatore, F.; Biffignandi, G. B.; Gaiarsa, S.; Rettani, M.; Bandi, C.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2129063
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