A fast and cost-effective procedure based on Inductively Coupled Plasma – Mass Spectrometry (ICP-MS) for the selective determination of methylmercury (MeHg) is proposed and validated for fish tissue analysis. Selectivity for MeHg is achieved by simply inserting a strong anion exchange resin to block inorganic mercury as negatively charged chloride species, leaving MeHg unretained. The procedure features a 15 min extraction time followed by a 100 s analysis time achieving a limit of detection of 1.6 μg kg−1 on solid samples. The effect of the solution composition and inorganic mercury concentration were extensively studied to fully assess the selectivity of the procedure: Hg(II):MeHg ratios up to 50 are tolerated and cause systematic errors lower than 15%. The entire procedure was successfully validated by standard reference material from the marine food web, namely fish muscle and liver plus zooplankton. The method was finally applied to the detection of MeHg in the marine trophic web of Djibouti (Gulf of Aden): a trophic magnification factor of 13.5 proved the high risk associated with the biomagnification of methylmercury.

Selective organomercury determination by ICP-MS made easy

Spanu D.
;
Butti L.;Boldrocchi G.;Bettinetti R.;Recchia S.
;
Monticelli D.
2022-01-01

Abstract

A fast and cost-effective procedure based on Inductively Coupled Plasma – Mass Spectrometry (ICP-MS) for the selective determination of methylmercury (MeHg) is proposed and validated for fish tissue analysis. Selectivity for MeHg is achieved by simply inserting a strong anion exchange resin to block inorganic mercury as negatively charged chloride species, leaving MeHg unretained. The procedure features a 15 min extraction time followed by a 100 s analysis time achieving a limit of detection of 1.6 μg kg−1 on solid samples. The effect of the solution composition and inorganic mercury concentration were extensively studied to fully assess the selectivity of the procedure: Hg(II):MeHg ratios up to 50 are tolerated and cause systematic errors lower than 15%. The entire procedure was successfully validated by standard reference material from the marine food web, namely fish muscle and liver plus zooplankton. The method was finally applied to the detection of MeHg in the marine trophic web of Djibouti (Gulf of Aden): a trophic magnification factor of 13.5 proved the high risk associated with the biomagnification of methylmercury.
2022
2022
Fish tissues; ICP-MS; Mercury speciation; Methylmercury; On-line separation; Trace element
Spanu, D.; Butti, L.; Boldrocchi, G.; Bettinetti, R.; Recchia, S.; Monticelli, D.
File in questo prodotto:
File Dimensione Formato  
Spanu et al Anal Chim Acta 2022.pdf

non disponibili

Tipologia: Versione Editoriale (PDF)
Licenza: Copyright dell'editore
Dimensione 1.38 MB
Formato Adobe PDF
1.38 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2131760
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 9
social impact