Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation. © 2012 Macmillan Publishers Limited All rights reserved.

Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation

Papait R.;Sala L.;Zaza A.;
2012

Abstract

Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation. © 2012 Macmillan Publishers Limited All rights reserved.
cardiac fibroblasts; cardiac repair; cardiomyocytes; iPS cells; Animals; Bone Morphogenetic Protein 2; Calcium; Cell Differentiation; Cells, Cultured; Cellular Reprogramming; Embryonic Stem Cells; Gene Expression Regulation; Induced Pluripotent Stem Cells; Karyotyping; Kruppel-Like Factor 4; Kruppel-Like Transcription Factors; Mice; Myocytes, Cardiac; Octamer Transcription Factor-3; SOXB1 Transcription Factors
Rizzi, R.; Di Pasquale, E.; Portararo, P.; Papait, R.; Cattaneo, P.; Latronico, M. V. G.; Altomare, C.; Sala, L.; Zaza, A.; Hirsch, E.; Naldini, L.; Condorelli, G.; Bearzi, C.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2133739
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