The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed. Finally, mice receiving a single systemic injection of Hu-mAb#33 were protected from death and severe clinical manifestations following both ocular and vaginal HSV-1 and -2 lethal challenge. These results pave the way for further studies reassessing the importance of HSV entry as a novel target for therapeutic intervention and inhibition of cell-to-cell virus transmission.

Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge

Massimo, Clementi;Nicasio, Mancini
2017-01-01

Abstract

The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed. Finally, mice receiving a single systemic injection of Hu-mAb#33 were protected from death and severe clinical manifestations following both ocular and vaginal HSV-1 and -2 lethal challenge. These results pave the way for further studies reassessing the importance of HSV entry as a novel target for therapeutic intervention and inhibition of cell-to-cell virus transmission.
2017
2017
https://www.sciencedirect.com/science/article/abs/pii/S0166354216307562?via=ihub
Herpes Simplex Virus; Cell-to-cell; Monoclonal antibody; In vivo infection
Clementi, Nicola; Criscuolo, Elena; Cappelletti, Francesca; Quaranta, Paola; Pistello, Mauro; A. Diotti, Roberta; A. Sutto, Giuseppe; W. Tarr, Alexander; Mailland, Federico; Concas, Daniela; Burioni, Roberto; Clementi, Massimo; Mancini, Nicasio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2149051
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