1. 1. Allogamus auricollis cell-free hemolymph proteins were analyzed by SDS-PAGE. Under reducing conditions the gel pattern showed two main components (83 and 76 kDa) and some lesser bands. 2. 2. After native PAGE, a single band showed phenoloxidase activity by in situ enzymatic staining. 3. 3. Spectrophotometric analysis of the cell-free plasma fraction was carried out with substrate and PTU as inhibitor.
Biochemical evidence of phenoloxidase activity (pro-PO system) in larvae of Allogamus auricollis (Insecta, Trichoptera)
Brivio M. F.
Primo
;
1992-01-01
Abstract
1. 1. Allogamus auricollis cell-free hemolymph proteins were analyzed by SDS-PAGE. Under reducing conditions the gel pattern showed two main components (83 and 76 kDa) and some lesser bands. 2. 2. After native PAGE, a single band showed phenoloxidase activity by in situ enzymatic staining. 3. 3. Spectrophotometric analysis of the cell-free plasma fraction was carried out with substrate and PTU as inhibitor.
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