Yeast artificial chromosomes (YACs) have recently provided a potential route to long-range coverage of complex genomes in contiguous cloned DNA. In a pilot project for 50 Mb (1.5% of the human genome), a variety of techniques have been applied to assemble Xq24-q28 YAC contigs up to 8 Mb in length and assess their quality. The results indicate the relative strength of several approaches and support the adequacy of YAC-based methods for mapping the human genome. © 1991.

Yeast artificial chromosome-based genome mapping: Some lessons from Xq24-q28

Porta G.;Palmieri G.;Romano G.;
1991-01-01

Abstract

Yeast artificial chromosomes (YACs) have recently provided a potential route to long-range coverage of complex genomes in contiguous cloned DNA. In a pilot project for 50 Mb (1.5% of the human genome), a variety of techniques have been applied to assemble Xq24-q28 YAC contigs up to 8 Mb in length and assess their quality. The results indicate the relative strength of several approaches and support the adequacy of YAC-based methods for mapping the human genome. © 1991.
1991
1991
Schlessinger, D.; Little, R. D.; Freije, D.; Abidi, F.; Zucchi, I.; Porta, G.; Pilia, G.; Nagaraja, R.; Johnson, S. K.; Yoon, J. -Y.; Srivastava, A.; Kere, J.; Palmieri, G.; Ciccodicola, A.; Montanaro, V.; Romano, G.; Casamassimi, A.; D'Urso, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11383/2169635
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