Fine anatomy and ultrastructural composition of adipose tissue is well known nowadays, On the other hand better understanding of adipose tissue ageing, especially in key district as face is a fundamental step for regenerative medicine and translational research in field such as plastic surgery. A lot of scientific studies have been published on the molecular and biochemical side of this matter, but less work has been performed on Extra cellular matrix collagen fibrils of face adipose tissue pads. This work aims to describe collagen organization, through different ages, in samples harvested from two fat areas of the face using Scanning and Transmission Electron Microscopy techniques and Light microscopy. During mayor maxillofacial surgery samples have been harvested from different patients aged 18 to 83 years old specifically Buccal Fat pad and deep malar fat pad. Fat samples have been fixed in Karnovsky. Later on, standard SEM, TEM and light Microscopy preparation was performed. Collagen fibrils organization in face adipose tissue can be described mainly by 2 patterns; The first is a thick mash of small collagen fibrils, known as a pericellular basket like-structure that we found in all the samples with low variability. The second pattern is represented by bundles of intercellular fibrils with same diameter. Collagen bundles are the structural element giving adipose tissue the specific mechanical and histological properties. Observation of Electron microscopy, both SEM and TEM, suggested that the quantity of intercellular collagen fibrils and bundles increase with growing age in both the fat-pads type analyzed. However, the organization and the cohesion of the collagen bundles gets lost as the age of the person increases. Taking into consideration lower magnification SEM pictures it is clear how the quantity of collagen increases whereas the bond between adipocytes decreases, therefore also the septa arrangement blends and gets less defined.
Ageing of facial fat, the role of extracellular collagen from an Electron microscopy point of view
Brambilla AndreaPrimo
;Protasoni MarinaSecondo
;Basso Petra Rita;Zammito RiccardoPenultimo
;Reguzzoni Marcella
Ultimo
2024-01-01
Abstract
Fine anatomy and ultrastructural composition of adipose tissue is well known nowadays, On the other hand better understanding of adipose tissue ageing, especially in key district as face is a fundamental step for regenerative medicine and translational research in field such as plastic surgery. A lot of scientific studies have been published on the molecular and biochemical side of this matter, but less work has been performed on Extra cellular matrix collagen fibrils of face adipose tissue pads. This work aims to describe collagen organization, through different ages, in samples harvested from two fat areas of the face using Scanning and Transmission Electron Microscopy techniques and Light microscopy. During mayor maxillofacial surgery samples have been harvested from different patients aged 18 to 83 years old specifically Buccal Fat pad and deep malar fat pad. Fat samples have been fixed in Karnovsky. Later on, standard SEM, TEM and light Microscopy preparation was performed. Collagen fibrils organization in face adipose tissue can be described mainly by 2 patterns; The first is a thick mash of small collagen fibrils, known as a pericellular basket like-structure that we found in all the samples with low variability. The second pattern is represented by bundles of intercellular fibrils with same diameter. Collagen bundles are the structural element giving adipose tissue the specific mechanical and histological properties. Observation of Electron microscopy, both SEM and TEM, suggested that the quantity of intercellular collagen fibrils and bundles increase with growing age in both the fat-pads type analyzed. However, the organization and the cohesion of the collagen bundles gets lost as the age of the person increases. Taking into consideration lower magnification SEM pictures it is clear how the quantity of collagen increases whereas the bond between adipocytes decreases, therefore also the septa arrangement blends and gets less defined.
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