The Ag(I) coordination polymer [Ag(mtpO)]n, containing the purine analog 1,2,4-triazolo[1,5-a]pyrimidin-7 (4H)-one (HmtpO) in its anionic form, has been synthesized and characterized in the solid state as per its thermal behaviour and spectroscopic properties, combining infrared, Raman, diffuse reflectance, and photoluminescence spectroscopy. Stability monitoring under simulated physiological conditions (DPBS, pH = 7.4, T = 37 degrees C) indicated a gradual and limited release of Ag+ ions, with their concentration increasing from 0.44 ppm after 1 h to 0.54 ppm after 7 days, thereby supporting the high water stability of [Ag(mtpO)]nand its potential for controlled Ag+ delivery. In view of its potential future applications in the treatment of microbial infections, [Ag(mtpO)]n was screened for in vitro cytotoxicity against a human dermal fibroblast (HDF) cell line. Importantly, the gradual release of Ag+ ions resulted in a lack of toxicity of the coordination polymer up to a concentration of 100 mu g mL-1 after 24 h treatment of normal cells. [Ag(mtpO)]nwas then tested for antimicrobial activity against bacteria (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 10145, Staphylococcus aureus ATCC 25923) and yeast (Candida albicans ATCC 10231) strains, in comparison with HmtpO and the known antibacterial agent AgNO3. Finally, the bioapplication studies of [Ag(mtpO)]nwere further extended to determine the inhibition of biofilm formation using the crystal violet assay.
A triazolopyrimidine Ag(I) coordination polymer: From solid-state characterization to bioapplication
Galli S.;
2026-01-01
Abstract
The Ag(I) coordination polymer [Ag(mtpO)]n, containing the purine analog 1,2,4-triazolo[1,5-a]pyrimidin-7 (4H)-one (HmtpO) in its anionic form, has been synthesized and characterized in the solid state as per its thermal behaviour and spectroscopic properties, combining infrared, Raman, diffuse reflectance, and photoluminescence spectroscopy. Stability monitoring under simulated physiological conditions (DPBS, pH = 7.4, T = 37 degrees C) indicated a gradual and limited release of Ag+ ions, with their concentration increasing from 0.44 ppm after 1 h to 0.54 ppm after 7 days, thereby supporting the high water stability of [Ag(mtpO)]nand its potential for controlled Ag+ delivery. In view of its potential future applications in the treatment of microbial infections, [Ag(mtpO)]n was screened for in vitro cytotoxicity against a human dermal fibroblast (HDF) cell line. Importantly, the gradual release of Ag+ ions resulted in a lack of toxicity of the coordination polymer up to a concentration of 100 mu g mL-1 after 24 h treatment of normal cells. [Ag(mtpO)]nwas then tested for antimicrobial activity against bacteria (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 10145, Staphylococcus aureus ATCC 25923) and yeast (Candida albicans ATCC 10231) strains, in comparison with HmtpO and the known antibacterial agent AgNO3. Finally, the bioapplication studies of [Ag(mtpO)]nwere further extended to determine the inhibition of biofilm formation using the crystal violet assay.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
