We have optimized the induction of antiviral cytotoxic T lymphocytes (CTL) in rhesus macaques by a lipopeptide vaccine containing seven peptides from simian immunodeficiency virus (SIV) Nef and Gag proteins and a strong T-helper peptide from tetanus toroid (TT) that is promiscuous in humans (peptide TT 830-846). Two of the eight immunized macaques showed T-helper (Th) cell proliferation and a specific synthesis of gamma interferon in response to TT 830-846 peptide. They also showed multispecific cytotoxic activity against three to five of the immunizing SN peptides. These results show the importance of a strong specific type 1 Th response for inducing a multispecific CTL response in vivo, which is essential for the development of an anti-human immunodeficiency virus vaccine.
|Data di pubblicazione:||1999|
|Titolo:||Type 1 CD4+ T-cell help is required for induction of antipeptide multispecific cytotoxic T lymphocytes by a lipopeptide vaccine in rhesus macaques.|
|Rivista:||JOURNAL OF VIROLOGY|
|Codice identificativo ISI:||WOS:000079701100106|
|Codice identificativo Scopus:||2-s2.0-0032899613|
|Codice identificativo Pubmed:||10196344|
|Appare nelle tipologie:||Articolo su Rivista|