Human basophils and mast cells express the chemokine receptor CCR3, which binds the chemokines eotaxin and RANTES. HIV-1 Tat protein is a potent chemoattractant for basophils and lung mast cells obtained from healthy individuals seronegative for Abs to HIV-1 and HIV-2. Tat protein induced a rapid and transient Ca21 influx in basophils and mast cells, analogous to b-chemokines. Tat protein neither induced histamine release from human basophils and mast cells nor increased IL-3-stimulated histamine secretion from basophils. The chemotactic activity of Tat protein was blocked by preincubation of FceRI1 cells with anti-CCR3 Ab. Preincubation of Tat with a mAb anti-Tat (aa 1–86) blocked the migration induced by Tat. In contrast, a mAb specific for the basic region (aa 46–60) did not inhibit the chemotactic effect of Tat protein. Tat protein or eotaxin desensitized basophils to a subsequent challenge with the autologous or the heterologous stimulus. Preincubation of basophils with Tat protein up-regulated the level of CCR3 mRNA and the surface expression of the CCR3 receptor. Tat protein is the first identified HIV-1-encoded b-chemokine homologue that influences the directional migration of human FceRI1 cells and the expression of surface receptor CCR3 on these cells.

Tat protein is an HIV-1 encoded SS-chemochine homolog that promotes migrationand upregulates CCR3 on human FceRI+ cells

TOSI, GIOVANNA;ACCOLLA, ROBERTO;
2000

Abstract

Human basophils and mast cells express the chemokine receptor CCR3, which binds the chemokines eotaxin and RANTES. HIV-1 Tat protein is a potent chemoattractant for basophils and lung mast cells obtained from healthy individuals seronegative for Abs to HIV-1 and HIV-2. Tat protein induced a rapid and transient Ca21 influx in basophils and mast cells, analogous to b-chemokines. Tat protein neither induced histamine release from human basophils and mast cells nor increased IL-3-stimulated histamine secretion from basophils. The chemotactic activity of Tat protein was blocked by preincubation of FceRI1 cells with anti-CCR3 Ab. Preincubation of Tat with a mAb anti-Tat (aa 1–86) blocked the migration induced by Tat. In contrast, a mAb specific for the basic region (aa 46–60) did not inhibit the chemotactic effect of Tat protein. Tat protein or eotaxin desensitized basophils to a subsequent challenge with the autologous or the heterologous stimulus. Preincubation of basophils with Tat protein up-regulated the level of CCR3 mRNA and the surface expression of the CCR3 receptor. Tat protein is the first identified HIV-1-encoded b-chemokine homologue that influences the directional migration of human FceRI1 cells and the expression of surface receptor CCR3 on these cells.
DE PAULIS, A.; DE PALMA, R.; DI GIOIA, L.; Carfora, M.; Prevete, N.; Tosi, Giovanna; Accolla, Roberto; Marone, G.
File in questo prodotto:
File Dimensione Formato  
De paulis 2000.pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: DRM non definito
Dimensione 1.03 MB
Formato Adobe PDF
1.03 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11383/1486294
Citazioni
  • ???jsp.display-item.citation.pmc??? 16
  • Scopus 73
  • ???jsp.display-item.citation.isi??? 66
social impact