In this study, the effect of naphthalene on the induction of apoptosis and gene expression profiles in cord blood cells has been evaluated, as well as its activity on the BCL-2 related protein expression. After 6, 24, and 48 h of exposure to naphthalene (500 μM , a decrease in cell death was observed: the cells became more resistant to the toxicant and capable of surviving after the treatment. A Western blot analysis revealed an overexpression of BCL-2, c-JUN, c-FOS, and RAF-1 proteins, which are involved in the antiapoptotic response and in the regulation of cell growth, differentiation, and development. Furthermore, macroarray analysis showed that naphthalene modified cord blood gene expression, inducing IL-8 precursor and T-cell transcription factor and decreasing the level of RNA-binding protein FUS/TLS.
|Titolo:||Naphthalene exposure: Effects on gene expression and proliferation in human cord blood cells|
|Autori interni:||ACQUATI, FRANCESCO|
|Data di pubblicazione:||2003|
|Rivista:||JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY|
|Appare nelle tipologie:||Articolo su Rivista|